Figure 7 .
GIS1 and MSN2/4 differentially affect the ability of manganese to protect cytosolic but not mitochondrial Fe/S proteins. The indicated strains were grown in SC medium that was supplemented for 6 hr with the designated concentrations of MnCl2 prior to cell lysate preparation and analysis of (A) aconitase and (B) isopropylmalate isomerase (IPMI) activity as described in Materials and Methods. Asterisk denotes a P < 0.05 and reflects the statistical significance between 4 mM Mn treatment and 0 or 2 mM Mn treatment. All reported values are averages based on triplicate cultures, with the errors representing the standard deviations. Strains employed: WT, BY4741; sod1∆ sod2∆, AR142; sod1∆ sod2∆ gis1∆, AR161; sod1∆ sod2∆ msn2/4∆, AR160.