Table 1. Growth phenotypes of wild-type and mutant strains.
Strain, genotype | APM concentration | Temperature | Pronamide concentration | |||||
---|---|---|---|---|---|---|---|---|
0.5 mM | 1.0 mM | 1.5 mM | 2.0 mM | 23° | 33° | 6 mM | 12 mM | |
CC-1690 (21gr) mt+ | 0 | 0 | 0 | 0 | +++ | +++ | +/0 | 0 |
CC-124 mt− | +/0 | 0 | 0 | 0 | +++ | +++ | +/0 | 0 |
CC-4432 apm1-1 | +++ | +++ | +++ | ++/0 | +++ | +++ | +++ | +++ |
CC-4433 apm1-5 | +++ | +++ | +/0 | 0 | +++ | +++ | +++ | +++ |
CC-4434 apm1-6 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
CC-4435 apm1-12 | ++ | +/0 | +/0 | +/0 | ++ | 0a | +/0 | 0 |
CC-4263 apm1-19 mt+ | +++ | +++ | +++ | ++/0 | +++ | +++ | +++ | +++ |
CC-4171 apm2-1A mt− | +++ | ++/0 | ++/0 | 0 | +++ | 0a | +++ | +++ |
CC-4172 apm2-1A mt+ | +++ | +++ | +/0 | 0 | +++ | 0a | +++ | ++ |
CC-4173 apm2-1B mt− | +++ | +/0 | 0 | 0 | +++ | 0a | +/0 | 0 |
CC-4174 apm2-2 mt+ | ++/0 | +/0 | +/0 | 0 | +++ | 0a | ++/0 | ++/0 |
CC-4175 apm2-2 mt− | ++/0 | +/0 | +/0 | 0 | +++ | 0a | ++/0 | ++/0 |
0, swollen, dead cells fail to divide; +, some normal-sized dividing cells; ++, many normal sized, dividing cells; +++, mostly normal sized, dividing cells.
Phenotype requires low plating density.