Table 2. Mapping the apm1 mutation.
| Locus or position on v4.0 chr 17 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| APMa | ACE 9390 | ACE 7549 | ACE 4477 | MSsc17 (FAP47) | ACE 9074 | ODA3 | ACE 4954 | C170199 | STSsc17 1016415 | ACE 5892 | C170104 | ACE 9357 | |
| 1401800 | 1299500 | 119500 | 1139000 | 1114000 | 1079000 | 992800 | 950900 | 890500 | 839700 | 779100 | 763500 | ||
| Progeny from cross A: apm1-19 × S1-D2 | |||||||||||||
| JF0204-E7 | 1b | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 |
| JF0105-A8 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-B10 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-D4 | 1 | 2 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
| JF3105-E9 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-G9 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| JF3105-G9 | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-G2 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-D7 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF0105-D11 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF3105-B6 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 1 | 0 | 1 |
| JF3205-E5 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| JF3205-F1 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Progeny from cross B: apm1-19 oda3 × S1-D2 | |||||||||||||
| JF0306-A5 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | |||||
| JF0306-E3 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | |||||
| JF0506-F9 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | |||||
C. reinhardtii apm1-19 mutant strains were crossed with the polymorphic strain S1-D2 to generate mapping progeny, each of which was derived from a separate tetrad.
a
Mapping progeny were scored for the APM1 phenotype by growth on 1 μM APM; genotypes for SNP markers were scored based on PCR products.
b
The C. reinhardtii allele is indicated by 1; the S1-D2 allele is indicated by 2; 0 indicates not scored.