Figure 6 .

Phenotypic and molecular characterization of transgenic kernels from functional complementation experiment. (A) The phenotype of 20 vitreous kernels and 10 opaque kernels, randomly selected from a transgenic line with homozygous o7 locus on chromosome 10. (B) Genotyping the 30 kernels displayed in A for o7 locus on chromosome 10 using the molecular marker Indel6819. All 30 kernels are homozygous at the o7 locus on chromosome 10. (C) Characterization of transgenic status of the 30 kernels displayed in A using the molecular marker Trans6837F3R3. The vitreous kernels (lanes 1–20) are transgenic, and the opaque kernels (lanes 21–30) are nontransformed. −, H₂O control; +, pTF102–O7Gene vector. (D) SDS–PAGE analysis of total proteins from different kernels. T+, transgenic and o7/o7 homozygous kernel; T−, nontransformed and o7/o7 homozygous kernel. (E) Comparison of zein protein content in wt, o7, pApB, transgenic homozygote o7/o7 (T+), and nontransformed homozygote o7/o7 kernels (T−).