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Two-hybrid interactions between the conserved region of Rpb1 foot with Mvp1 and Spo14. (A) Conserved region of Rpb1 foot fused to Gal4BD in plasmid pGBT9 was tested against pACT2–Mvp1 and pACT2–Spo14. β-Galactosidase was tested in an overlay assay (Flores et al. 1999). +, positive control for interaction (Rpb5 and a region of Rpa190). (B) Summary of the interactions.
