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. 2011 Dec;189(4):1235–1248. doi: 10.1534/genetics.111.133215

Figure 9 .

Figure 9 

Deletion of MVP1 or SPO14 affects the amount of phosphorylated RNA pol II in vivo. ChIP analysis of Rpb1, CTD Ser5P, and CTD Ser2P was performed in wild-type (WT) and Δmvp1 and Δspo14. Binding to promoter or 5′ region (A) and 3′ region (B and C) of MBF1, RPS15, PMA1, HSP26, HSP12, and PYK1 genes were analyzed by quantitative RT–PCR. Numbers on the Y-axis represent the percentage of Rpb1 and Rpb1–CTDSer5P or Rpb1–CTDSer2P cross-linked to the DNA region in Δmvp1 and Δspo14 cells relative to WT cells, where cross-linking is considered 100%. (D) Analysis of genetic interactions between Δmvp1, Δspo14, and Δrtr1 mutants (top) or Δmvp1, Δspo14, and ceg1-250 mutants (bottom). *P < 0.05, **P < 0.01, ***P < 0.001.