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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Sep 22;31(12):e100–e106. doi: 10.1161/ATVBAHA.111.238261

Figure 5. Co-immunoprecipitation of PAR1 with PAR2.

Figure 5

(top) T7-PAR1 was transiently co-expressed in COS7 cells with PAR2-myc or pcDEF3 vector alone (-) as indicated. T7 agarose beads were used to immunoprecipitate bound PAR2-myc from cell lysates as shown by the myc immunoblot. (middle) Immunoblot of T7 confirmed the presence of T7-PAR1, (bottom) and β-actin staining confirmed equal loading of the lysates.