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. Author manuscript; available in PMC: 2011 Dec 16.
Published in final edited form as: Nature. 2010 Oct 13;468(7323):562–566. doi: 10.1038/nature09513

Figure 1. Time course of cell generation and BBB development in the rat cerebral cortex.

Figure 1

a–g, Sections of rat cerebral cortex at indicated ages were stained for endothelial cells with Bandeiraea simplicifolia lectin I (BSL) (green, a–f) and nuclei with DAPI (blue, a, f (left), g), pericytes with anti-PDGFR-β (red, b; white arrows point to pericytes), oligodendrocyte progenitors with anti-PDGFR-α (red, c), astrocytes with anti-aquaporin 4 (red, d), anti-occludin (red, e; yellow arrows indicate tight-junction strands), anti-Glut1 (red, f (right)), and anti-Pgp (red, g). Scale bars represent 100 µm (a–d, f, g) and 20 µm (e). h–j, Rats aged E15 (left), E21 (middle) and adults (right) were given a trans-cardiac perfusion of biotin, and liver (h), muscle (i) and brain (j) tissue sections were stained with streptavidin (green) and DAPI (blue). Scale bar represents 100 µm.