Figure 5. GFP-tagged CaMKII δB and δC expressed in AMVMs isolated from δKO mice.
AMVMs isolated from δKO mice were infected with adenovirus expressing (A) GFP-tagged CaMKII δB or (B) GFP-tagged CaMKII δC. AMVMs were imaged using confocal microscopy, DAPI staining was used to identify the nuclei, and line scan quantification was used to measure fluorescence intensity and determine enzyme distribution. Scale bar is 15 microns (μm). Background measurements taken from non-infected AMVMs were averaged and subtracted from the fluorescence intensity measurements of the GFP-tagged enzyme.