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. Author manuscript; available in PMC: 2011 Dec 16.
Published in final edited form as: Nat Cell Biol. 2010 Mar 14;12(4):330–340. doi: 10.1038/ncb2034

Figure 6. Model of clathrin-, AP-1-coated carrier biogenesis.

Figure 6

(a) ARF1 activation triggers the recruitment of AP-1 and clathrin onto TGN membranes leading to cargo sorting. The CYFIP, ABI, NAP1 complex is recruited at the edges of the clathrin-, AP-1 coated subdomains of the TGN. HIP1R binding to clathrin light chains could prevent actin polymerization on the surface of clathrin coats. (b) RAC1, activated by its GEF β-PIX, which forms a complex with the ARF1-GAP GIT1 and/or GIT2, binds to CYFIP and activates the actin nucleation complex leading to N-WASP-dependent activation of ARP2/3 and actin polymerization towards the TGN membrane during the initial stages of tubular carrier formation. (c) BAR domain proteins, dynamin2 and cortactin bind to tubular membranes. These molecules can bind N-WASP and thus sustain ARP2/3-dependent actin polymerization during tubule elongation and fission.