. Author manuscript; available in PMC: 2011 Dec 16.

Published in final edited form as: Nat Cell Biol. 2010 Mar 14;12(4):330–340. doi: 10.1038/ncb2034

Table I. Biogenesis of GFP-MPR tail-containing tubular transport carriers in siRNA-treated HeLa cells.

GFP-MPR tail expressing cells were incubated with the indicated siRNAs for 72h. The exit of GFP-MPR tubules from the TGN region was monitored using time-lapse fluorescence microscopy. The interval between the acquisition of 2 consecutive images t = 500 ms. N = 5 independent experiments for siCYFIP2 and siCYFIP1 and n = 3 independent experiments for the other conditions; data represent the mean ± s.d.;

	No Tubules growing form the TGN/cell/2min	Tubule length (μm)	No Cells
siNon	4.3 ± 1.02	5.9 ± 2.8	40
siCYFIP2	1.72 ± 0.99^*	2 ± 0.55^*	50
siCYFIP1	1.53 ± 0.99^*		42
siN-WASP	2.07 ± 0.77^*	1.74 ± 0.43^*	25
siCDC42	3.05 ± 1.14	3.44 ± 1.61^*	47
siRAC1	1.98± 0.81^*	2.35 ± 0.81^*	100
siβ-PIX	1.83± 1.2^*	1.59± 0.42^*	36

p < 0.0001, Anova single factor analysis, α = 0.05.