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. 2011 Dec 16;6(12):e28574. doi: 10.1371/journal.pone.0028574

Figure 12. Expression of Netrin1 and Slit2.

Figure 12

(A) Whole mount in situ hybridization to detect Netrin1 expression in an E9 mouse embryo. Note the absence of signal in the telencephalic vesicles. (B–D) Photomicrographs illustrating Netrin1 in situ signal on sections of E11 (B) and E14 (C, D) mouse embryonic brains. Netrin1 mRNA is detected in the telencephalon of E14 embryos, but in E11 embryos, telencephalic vesicles are not labeled. (E–G) Photomicrographs showing Slit2 in situ signal on horizontal sections of E13 (E, F) and E14 (G) rat embryos. Slit2 is expressed early in the septal neuroeptithelium (E13) and in the hypothalamic neuroepithelium (E, F). At E14, Slit2 is expressed in preoptic and posterior hypothalamic neuroepithelium (G). Scale bar: A = 1 mm; B, E–G = 400 µm; C = 600 µm; D = 250 µm. CP: caudoputamen; CTX: cerebral cortex; Mes: mesencephalon; nh: neuroepithelium; septal nh: septal neuroepithelium; sopt: optic sulcus; SP: spinal cord; Tel: telencephalon; V3: third ventricle; VL: lateral ventricle.