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. 2011 Aug 3;39(21):9275–9282. doi: 10.1093/nar/gkr606

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Impact of proto-spacer and PAM mutations on CRISPR-encoded plasmid immunity. (A) Effect of mutations in the proto-spacer region on the plasmid transformation efficiency. Mutations are shown schematically above the figure. (B) Effect of mutations in the PAM region on the plasmid transformation efficiency.