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. 2011 Aug 16;39(21):9376–9389. doi: 10.1093/nar/gkr615

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Isolation of tRNA^Thr species and threonine charging activity. (A) tRNA species were isolated by solid-phase DNA probe column chromatography as described in Materials and methods section. The purified tRNA species were analyzed by 15% PAGE/7 M urea. The gel was stained with methylene blue. The tRNA^Thr3(UGU) was not obtained by the solid-phase DNA probe column chromatography. (B) Threonine charging activities of the isolated tRNAs were measured as described in ‘Materials and Methods’ section. Abbreviations used are as follows: Thr1, tRNA^Thr1(CGU); Thr2, tRNA^Thr2(UGU); Thr3, tRNA^Thr3(UGU); and Thr4, tRNA^Thr4(GGU).