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. 2011 Aug 16;39(21):9376–9389. doi: 10.1093/nar/gkr615

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Structure of AFU-CSL. (A) Ribbon diagram of the overall structure of AFU-EndA (orange, PDB ID code 1RLV). (B) The crystal structure of AFU-CSL (orange). Two dotted circles indicate the inserted CSL (red). (C) Left: Superimposed structures of archaeal EndAs. The α-subunits of APE-EndA are indicated in pink and light grey. The β-subunits of APE-EndA are indicated in green and cyan. The α-subunits of AFU-CSL are in orange. Right: Close-up view of the structure of CSL region (red) of AFU-CSL (orange) superimposed on the structure of the corresponding region of APE-EndA (pink). The RMSD is 2.6 Å for 628 Cα atoms of AFU-CSL. (D) Electrostatic potential surface models of AFU-EndA: red and blue colors indicate negative and positive charges, respectively. (E) Electrostatic potential surface models of AFU-CSL. Two dotted circles (yellow) show the K179 and R181 residues of AFU-CSL. (F) Electrostatic potential surface models of APE-EndA. Two dotted circles (yellow) show the K44 and R46 residues of APE-EndA.