Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 3;39(21):9306–9315. doi: 10.1093/nar/gkr619

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Binding experiments with tRNA^Gln, GluRS2 and GatCAB as shown by size-exclusion chromatography. (A) Comparison of isolated GluRS2 and GluRS2/tRNA^Gln complex profiles. The association enabled us to estimate a K_D value for GluRS2 and tRNA^Gln binding (see ‘Materials and Methods’ section). (B) Comparison between free and tRNA^Gln-complexed GatCAB. (C) Gel-filtration of a solution containing the three partners. (D) The same experiment as described in (C) was performed with preformed Glu-tRNA^Gln (grey line) or after incubation of the three partners within an aminoacylation medium containing free Glu and ATP, to provide endogenous Glu-tRNA^Gln (dotted line). Proteins and tRNA^Gln were added at concentrations of 20 µM each for the experiments shown in all panels.