Skip to main content
. 2011 Aug 16;39(21):9390–9404. doi: 10.1093/nar/gkr630

Figure 5.

Figure 5.

Association of RHAU with telomerase holoenzyme subunits. (A) TERC G4 motif dependent association of RHAU with dyskerin. Proteins from whole HEK293T cell lysates of either mock-transfected (−) cells or transiently expressing WT or G4 motif mutant (G4-MT, ΔG4) forms of TERC were immunoprecipitated with either control mouse IgGs (mIgG) or anti-dyskerin antibodies. Immunopurified RNP complexes were separated by SDS–PAGE and probed with anti-RHAU or anti-dyskerin antibodies. (B) TERC G4 motif-dependent association of RHAU with TERT. Protein immunoprecipitation experiments with anti-FLAG antibodies were performed with whole cells lysates of HEK293T cells transiently expressing TERT–FLAG protein together with WT or G4 motif mutant (G4-MT, ΔG4) forms of TERC. Immunopurified RNP complexes were separated by SDS–PAGE and probed with anti-RHAU or anti-FLAG antibodies. The asterisk denotes immunoprecipitated TERT–FLAG protein that cross-reacted unspecifically with the horsearadish peroxidase-conjugated secondary antibody. (C) Western blot analysis of comparable protein expression in input HEK293T cell lysates. (D) Protein immunoprecipitation experiments with anti-dyskerin antibodies were performed with whole cells lysates of HEK293T cells transiently expressing TERT–FLAG protein together with WT or G4 motif mutant (G4-MT, ΔG4) forms of TERC. Immunopurified RNP complexes were separated by SDS–PAGE and probed with anti-FLAG or anti-dyskerin antibodies.