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. 2011 Aug 8;39(21):9224–9237. doi: 10.1093/nar/gkr647

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — IR enhances the interaction between PNKP, XRCC4 and DNA ligase IV. C13 PNKP knockdown cells stably re-expressing vector (vec, lanes 1 and 2), shRNA-resistant wt HA-PNKP (lanes 3 and 4), HA-PNKP-S114A-S126A (2A, lanes 5 and 6) or HA-PNKP-S14D-S126D (2D, lanes 7 and 8) were either unirradiated (−) or irradiated 10 Gy (+) and harvested after 60 min. HA-PNKP was immunoprecipitated and resolved by SDS–PAGE as described above. Immunoblots were probed for HA (for PNKP), XRCC4 or DNA Ligase IV as indicated. Whole cell extract of 50 µg (input) was also analyzed for XRCC4 and DNA ligase IV levels (bottom two panels). See also Supplementary Figure S7.