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. 2011 Aug 11;39(21):9238–9249. doi: 10.1093/nar/gkr653

Figure 4.

Figure 4.

TWINKLE in combination with POLγ can support DNA replication on a circular dsDNA template. (A) The bubble template was prepared as described in ‘Materials and Methods’ section. The tentative localization of TWINKLE (light gray hexamer) and POLγ (dark gray and black heterotrimer) are indicated. The bubble region is symmetric and may contain one annealed primer on each strand, allowing initiation of DNA synthesis in opposite directions. (B) DNA replication assays were performed using the bubble template (10 fmol), POLγ (70 fmol), and increasing amounts of TWINKLE (0, 50, 200 and 750 fmol) for 60 min at 42°C. (C) DNA replication assays were performed as in (B) but in presence or absence of 750 fmol TWINKLE. The reactions were incubated for 0, 2, 15, 40 or 70 min at either 25°C (upper panel) or 42°C (lower panel). SM, size marker.