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. 2011 Aug 18;39(21):9413–9421. doi: 10.1093/nar/gkr663

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The linker region mediates cooperative binding interactions between ESE and SRSF1-RBD. (A) Filter-binding assay of His-tagged SRSF1-RBD wt and mutants, F56D/F58D from R1, W134A, Q135A and R154A from R2 to Ron ESE of 13 nt. Error bars obtained from three independent experiments. (B) Filter-binding assay of His-tagged SRSF1-RBD wt, and four different tyrosine to alanine mutants, Y79A and Y82A from R1, and Y149A and Y153A from R2 to 13-mer Ron ESE. Error bars obtained from three independent three experiments. (C) Filter-binding assay showing cooperative interactions between ESE and mixtures of R1 and different constructs of R2 (R2, LR2Δ5 and LR2) of SRSF1. Error bars obtained from three independent three experiments. (D) Filter-binding assay showing cooperation between the N-terminal and C-terminal of the linker of SRSF1 by using three different mutants (S91E/T95 from N-terminal, S116E/S119E from C-terminal, and S91E/T95E/S116E/S119E). The number in parenthesis denotes the apparent K_d of binding and (−) indicates not determined.