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Tm curves for duplexes of 5′-AUGCUXAGUCGA-3′ with complementary RNA (left column) and complementary DNA (right column) displaying all four natural nucleobases opposite X; top row: X = 5-nbnOrC; middle row: X = rC and bottom row: X = 5-HOrC; duplex conc.: 2 µM in 10 mM NaH2PO4, 150 mM NaCl, pH 7.0.
