Figure 2.

HPLC purification of RNA identifies contaminants eluting before and after the expected product. Chromatogram of Ψ-modified TEVeGFPA_n mRNA. RNA was applied to the HPLC column and eluted using a linear gradient of Buffer B (0.1 M TEAA, pH 7.0, 25% acetonitrile) in Buffer A (0.1 M TEAA, pH 7.0). The gradient spanned 38–55% Buffer B over 22 min (red line). Absorbance at 260 nm was analyzed (black line), which demonstrated the expected sized RNA as well as smaller and larger RNA species. Data shown are from one experiment that is representative of over 200.