Figure 1.

Schematic of proof of principle evaluation with NOX-E36-Int. 5′–fragments in this schematic have been encompassed using the formula ‘R’-NH-(CH₂)₆-OP(O)(OH)-G(X)_ycp where ‘R’ is either the structurally drawn cleavable biotin affinity tag or cleaved fragment. Letters in bold and underlined, respectively, represent the nucleic acid sequence, X indicates the identity of the particular nucleotide (A, C, G, U) read from the sequence 5′- to 3′- and y represents how many additional nucleotides are in the fragment over the first fragment. For example, for fragment 2: y = 1 (one extra nucleotide). The extra nucleotide(s) X over the first fragment is one (C) therefore the fragment, fragment 2 is ‘R’-NH-(CH₂)₆-OP(O)(OH)-GCcp. The annotation cp in subscript denotes 2′3′ cyclic phosphate. The l-RNA sequence of the Spiegelmer is in bold letters. FLP = Full Length (undegraded) Product.