Table 2.
Sequence confirmation of NOX-E36 Int
| Fragmenta/RT (min) | Structureb | Observed (Da) | Calculated (Da) |
|---|---|---|---|
| 1/3.57 | R-Gcp | 612.12 | 612.12 |
| 2/4.85 | R-GCcp | 917.16 | 917.16 |
| 3/8.65 | R-GCAcp | 1246.21 | 1246.21 |
| 4/11.49 | R-GCACcp | 1551.25 | 1551.25 |
| 5/14.53 | R-GCACGcp | 1896.30 | 1896.30 |
| 6/16.99 | R-GCACGUcp | 2202.32 | 2202.32 |
| 7/18.91 | R-GCACGUCcp | 2507.36 | 2507.37 |
| 8/20.53 | R-GCACGUCCcp | 2812.40 | 2812.41 |
| 9/21.88 | R-GCACGUCCCcp | 3117.44 | 3117.45 |
| 10/23.07 | R-GCACGUCCCUcp | 3423.46 | 3423.47 |
| 11/24.08 | R-GCACGUCCCUCcp | 3728.51 | 3728.52 |
| 12/25.50 | R-GCACGUCCCUCAcp | 4057.55 | 4057.57 |
| 13/26.13 | R-GCACGUCCCUCACcp | 4362.60 | 4362.61 |
| 14/26.81 | R-GCACGUCCCUCACCcp | 4667.64 | 4667.65 |
| 15/27.73 | R-GCACGUCCCUCACCGcp | 5012.68 | 5012.70 |
| 16/28.67 | R-GCACGUCCCUCACCGGcp | 5357.72 | 5357.75 |
| 17/29.52 | R-GCACGUCCCUCACCGGUcp | 5663.75 | 5663.77 |
| 18/30.53 | R-GCACGUCCCUCACCGGUGcp | 6008.81 | 6008.82 |
| 19/31.45 | R-GCACGUCCCUCACCGGUGCcp | 6313.85 | 6313.86 |
| 20/33.25 | R-GCACGUCCCUCACCGGUGCAcp | 6642.90 | 6642.91 |
| 21/34.91 | R-GCACGUCCCUCACCGGUGCAAcp | 6971.96 | 6971.96 |
| 22/35.84 | R-GCACGUCCCUCACCGGUGCAAGcp | 7317.01 | 7317.01 |
| 23/36.72 | R-GCACGUCCCUCACCGGUGCAAGUcp | 7623.05 | 7623.04 |
| 24/37.79 | R-GCACGUCCCUCACCGGUGCAAGUGcp | 7968.13 | 7968.08 |
| 25/39.53 | R-GCACGUCCCUCACCGGUGCAAGUGAcp | 8297.15 | 8297.14 |
| 26/41.13 | R-GCACGUCCCUCACCGGUGCAAGUGAAcp | 8626.19 | 8626.19 |
| 27/41.81 | R-GCACGUCCCUCACCGGUGCAAGUGAAGcp | 8971.29 | 8971.24 |
| 28/42.51 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCcp | 9276.31 | 9276.28 |
| 29/43.26 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCcp | 9581.24 | 9581.32 |
| 30*/44.18 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGcp | 9931.04 | 9931.02 |
| 31*/44.89 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUcp | 10237.26 | 10237.19 |
| 32*/45.69 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGcp | 10582.54 | 10582.40 |
| 33*/46.43 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGcp | 10927.70 | 10927.60 |
| 34*/47.05 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCcp | 11232.86 | 11232.78 |
| 35*/47.65 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUcp | 11538.97 | 11538.95 |
| 36*/48.23 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUCcp | 11844.12 | 11844.13 |
| 37*/48.71 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUCUcp | 12150.36 | 12150.29 |
| 38*/49.06 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUCUGcp | 12495.72 | 12495.50 |
| 39*/49.79 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUCUGCcp | 12800.76 | 12800.68 |
| FLP*/49.06 | R-GCACGUCCCUCACCGGUGCAAGUGAAGCCGUGGCUCUGCG | 13083.85 | 13083.92 |
This table shows the retention time, structure and deconvoluted observable masses (exact or average) obtained from the TIC along with the corresponding calculated values of the expected fragments.
aFragment number (in bold) corresponds to fragment size according to the sequence.
bStructure and sequence of fragments: R = HS-(CH2)2C(O)-NH(CH2)6-OP(O)(OH)-, cp in subscript annotation denotes 2′3′–cyclic phosphate, l-RNA sequence of Spiegelmer is in bold letters; FLP = Full Length (undegraded) Product.
All masses observed are exact isotopic masses unless indicated (*) whereby the observed mass is the corresponding average molecular mass.
Calculated masses are switched to average molecular weight accordingly.