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. 2011 Dec 16;6(12):e28976. doi: 10.1371/journal.pone.0028976

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Moelants et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chemokines [CXCL8 (♦)], citrullinated chemokines [[Cit⁵]CXCL8 (▪) and [Cit⁹]CXCL5 (•)] and RPMI 1640 medium containing 2% FBS [control medium (x)] were chemically modified with antipyrine and 2,3-butanedione at low pH. Subsequently, samples were dialyzed against a buffer with neutral pH and modified citrullinated CXCL8 ([Cit⁵]CXCL8*) was detected by ELISA using anti-CXCL8 coating antibodies and detection antibodies against modified citrulline residues. Untreated samples (Panel A) and samples treated with antipyrine and 2,3-butanedione (Panel B) are represented as dotted and full lines respectively.