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. 2011 Dec 16;6(12):e28976. doi: 10.1371/journal.pone.0028976

Figure 6. ELISA standard curves for citrullinated CXCL8 in various biological fluids.

Figure 6

Standard curves for [Cit5]CXCL8 were generated in culture medium containing 10% FBS or in human plasma, serum or whole blood. The indicated concentrations of [Cit5]CXCL8 represent the initial concentrations spiked in the complex samples before chemical modification and dialysis. Panel A shows two independent standard curves for [Cit5]CXCL8, standard 1 (○) and 2 (•), in RPMI 1640 medium containing 10% FBS. For both standards the detection range is 1–50 ng/ml of [Cit5]CXCL8 in the original sample. In a second series of experiments (Panel B) standard curves for [Cit5]CXCL8 were generated in RPMI 1640 medium containing 10% FBS (•) or in human plasma (▪), serum (▴) or whole blood (♦) (average of two independent experiments).