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. 2011 Dec 16;6(12):e28981. doi: 10.1371/journal.pone.0028981

Figure 4. AMPK-mediated FAT/CD36 translocation in Control cells does not modify lipid accumulation.

Figure 4

A. Quantification of lipid accumulation in Control (n = 4) (A) after palmitate treatment (0.6 mM for 16 h) or palmitate treatment plus AICAR stimulation (500 µM for 1 h) or palmitate treatment after SSO addition (250 µg/ml for 30 min) or palmitate treatment with or without AICAR stimulation after SSO addition. Data are means ±SEM. Each point was assayed in triplicate for each of the 4 independent cell cultures. B. Representative fluorescence and light microscopy of myotubes established from control subjects (Control) and from obese type 2 diabetic patients (OBT2D) after 8 days of differentiation infected with an adenovirus expressing either GFP (GFP) or GFP and the constitutively activated form of AMPK alpha 2 (alpha 2) stained by oil red O (ORO) after palmitate treatment (0.6 mM for 16 h). C. Palmitate beta-oxidation in differentiated Control cells (n = 3) before (−) and after (+) AICAR stimulation (500 µM for 1 h) expressed relative to protein content. Experiments were performed in triplicate for each of the 3 independent cell cultures. Data are mean ±SEM. *, P<0.05, AICAR-treated versus untreated Control cells.