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. 2011 Dec 16;6(12):e29061. doi: 10.1371/journal.pone.0029061

Figure 1. Characterization of the double band corresponding to dysferlin observed in CD14+ PBM by WB.

Figure 1

A) Dysferlin siRNA efficiently reduced both dysferlin bands in PBM. Representative WB of monocytes from a healthy donor treated with siRNA showed that siDYSF efficiently reduced the two bands corresponding to dysferlin. On the right, quantification of WB from silenced monocyte samples, corresponding to four independent experiments, showed that when dysferlin was knocked down, levels diminished from 100% in Control (C) and 104.2±11.3% in siMYOF to 58.4±14.8% with siDYSF1 (p<0.05) and 72.2±9.8% with siDYSF2 (p<0.05), error bars indicate standard desviation. B) Schematic overview of the dysferlin protein and the epitopes recognized by F4, H7 and Hamlet antibodies. The three affinity binders together cover the complete dysferlin protein sequence. C) Both F4 and H7 antibodies can immunoprecipitate the dysferlin doublet from PBM. Bound (B) and non-bound (NB) fractions were analyzed by WB for dysferlin. The arrow denotes the dysferlin doublet observed in the bound fraction using F4 and H7 and in the non-bound fraction using an antibody against β-amyloid.