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. 2011 Dec 16;6(12):e29174. doi: 10.1371/journal.pone.0029174

Figure 7. Analysis of pre-rRNAs intermediates in HeLa and HEK293 Flp-In T-Rex cells transiently transfected with a siRNA targeting the FTSJ3 mRNA.

Figure 7

(A) Western blot analysis to determine FTSJ3 levels 72 h following siRNA transfection. (B) Northern blot using probe P3 complementary to ITS1 upstream site 2b. (C) Graphs representing the ratio between the 34S and 47S pre-rRNA of the Northern blots shown in B. Bands were quantified by using ImageQuant software. Control cells were transfected with siRNA targeting the GFP mRNA.