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. 2011 Dec 16;6(12):e29318. doi: 10.1371/journal.pone.0029318

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Mitroulis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PMNs from control subjects treated with MSU crystals for different time points. NETs are assessed by immunofluorescence microscopy after co-staining with DAPI (blue) and MPO (green). Control represents untreated PMNs incubated for 180 min. Scale bars, 30 µm. Original magnification 400x. One out of three independent experiments is shown. B) Percentage of NET releasing cells presented in panel A. Data are representative of three independent experiments and presented as mean ± SD. * P<0.05; ns = non significant compared to control cells.