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. 2011 Dec 16;6(12):e29318. doi: 10.1371/journal.pone.0029318

Figure 2. PI3K signaling and endosomal acidification is involved in NET release after treatment with MSU crystals.

Figure 2

A) Treatment with 3-MA or bafilomycin A prevents the release of NETs from PMNs from control subjects treated with MSU crystals for 180 min. NETs are determined by co-staining with DAPI (blue) and MPO (green) and visualized by immunofluorescence microscopy. Scale bars, 30 µm. Original magnification 400x. One out of four independent experiments is shown. B) The effect of treatment with 3-MA, LY294002, bafilomycin A or PP1 in the percentage of NET releasing PMNs incubated with MSU crystals. Data are representative of four independent experiments and presented as mean ± SD. * P<0.05; ns = non significant compared to MSU treated cells.