Skip to main content
. 2011 Dec 16;6(12):e29318. doi: 10.1371/journal.pone.0029318

Figure 3. Induction of autophagy in PMNs treated with MSU crystals.

Figure 3

A) Induction of autophagy in control PMNs treated with MSU crystals for 20 min and 60 min, as assessed by immunofluorescence microscopy of endogenous LC3B. LC3B-positive autophagosome formation was blocked by 3-MA. DNA is labeled with DAPI (blue). LC3B was stained with polyclonal anti-LC3B Ab (green). Scale bars, 5 µm. Original magnification 1000x. One out of three independent experiments is shown. B) Induction of LC3B-I to LC3B –II conversion in PMNs treated with MSU crystals for 45 min, as assessed by immunoblotting. Attenuation of LC3B conversion in PMNs treated with 3-MA. C) Measurement of integrated optical density (IOD) of bands presented in B, expressed as LC3B-II to LC3B-I ratio ± SD. Representative data from four independent experiments are shown in B and C. * P<0.05 compared to MSU treated cells.