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. 2011 Dec 16;6(12):e29318. doi: 10.1371/journal.pone.0029318

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Mitroulis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) NET formation by synovial cells, peripheral PMNs from patients with gout and from control PMNs treated with synovial fluid or serum from patients with gout, as assessed by immunofluorescense microscopy after co-staining with DAPI (blue) and MPO (green). Inhibitory effect of treatment with 3-MA on the release of NETs from synovial cells. Scale bars, 30 µm. Original magnification 400x. One out of six independent experiments is shown. B) The effect of treatment with 3-MA or bafilomycin A in the percentage of NET releasing cells presented in panel A. Data are representative of six independent experiments and presented as mean ± SD. ‡ P<0.05 compared to control, * P<0.05.