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. 2011 Dec 12;195(6):979–992. doi: 10.1083/jcb.201106098

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© 2011 Bruns et al.

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Figure 3. — Grh1-GFP does not colocalize with marker proteins of the Golgi apparatus and endosomes under normal growth conditions or upon starvation. (A and B) Grh1-GFP was coexpressed with the early Golgi marker proteins CopI-RFP and AnpI-RFP or the late Golgi marker protein Sec7-DsRed. Cells were cultured in growth conditions (A) or nutrient starved for 4 h (B) and visualized by fluorescence microscopy. (C and D) Grh1-GFP was coexpressed with mCherry-Tlg1 or mCherry-Pep12 in growth conditions (C) or nutrient starved (D) and visualized by fluorescence microscopy. (E) Grh1-GFP–expressing cells were labeled with FM 4-64 and transferred to starvation medium for up to 3 h to visualize the localization of the internalized FM 4-64 with reference to Grh1-GFP. Bar, 2 µm.