. 2011 Dec;8(4):167–179. doi: 10.1089/zeb.2011.0704

Table 2.

Comparison of Protocols for Sperm Cryopreservation of Green Swordtail

	Conventional cryopreservation^a	Vitrification^b
% cryoprotectant	14% Gly	20%EG+20%Gly
Exposure time	10–120 min	<1 min
Apparatus	0.25-mL straw	5-mm loops
Sample volume	80–220 μL	∼10 μL
Cooling rate	20–30°C/min	50,000–720,000°C/min^c
Thawing rate	1300–2500°C/min^d	200,000°C/min

^{^a}

Yang and Tiersch.⁹

^{^b}

This study.

^{^c}

Isachenko et al.³⁸

^{^d}

Rall and Fahy.⁵⁹