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. 2011 Nov 21;108(49):19629–19634. doi: 10.1073/pnas.1117544108

Fig. 4.

Fig. 4.

Vorinostat, UCN-01, and in combination induce abnormal mitosis. Representative mitotic spreads of (A) HFS, (B) LNCaP, and (C) A549 cells in 24 h culture without (control) or with indicated inhibitors: 5 μM vorinostat (V), 400 nM UCN-01 (U), vorinostat plus UCN-01 (V + U). (Scale bar, 10 μm.) (D) Percentage of metaphase spreads with the indicated aberration in LNCaP and A549 cells cultured with indicated inhibitors (n > 150 cells). (E) Aberrations per metaphase in LNCaP and A549 cells cultured with indicated inhibitor. (F) Immunoblots of phosphorylated H3 (p-H3) of HFS, LNCaP, and A549 cells cultured with indicated inhibitors for 24 h. Histone H3 is a loading control. (G) Representative mitotic spreads HFS and LNCaP cells cultured with vorinostat for 24 h followed by 24 h cultured without inhibitor. (Scale bar, 10 μm.) (H) Cell density in cultures of HFS and LNCaP for 72 h with indicated inhibitors, then cultured without inhibitors for indicated times. “0 h” is time of medium replacement at 72 h. Viable cells were measured by trypan blue staining. Data are represented as mean ± SD. *P < 0.05, ***P < 0.001. P value was derived from two-way ANOVA.