Fig. 3.

V. cholerae biofilm genes are strongly induced inside the fly. Results from three independent experiments measuring the transcription of the vpsL gene in flies after ingestion of V. cholerae in PBS for 48 h relative to V. cholerae resuspended in PBS for 2 h are shown. Each experiment was performed in triplicate. Normalization was performed with the clpXP gene, and fold change was calculated using the 2^−ΔΔCt method.