Fig. 3.

In vivo targeting of IF7-A488 to tumors. (A) LLC tumors were allowed to become vascularized for 3 d in a dorsal skinfold chamber window in nude mice (21). Mice were injected with 100 μL of 50 μM IF7-A488 (a) or RQ7-A488 (b) in 5% glucose in water. Green fluorescence signals were recorded by video under a fluorescence microscope up to 40 min postinjection. IF7-A488 was also injected into tumor-bearing mice preinjected with rabbit IgG (c) or with rabbit anti-Anxa1 (N-19) antibody (d). From left: bright field before injection, and fluorescence at 0, 5, and 20 min after IF7-A488 injection. Far right boxes indicate the boundary between the tumor and stroma at 20 min (Scale bar,500 μm). (B) Quantitative analysis of fluorescence during the course of the analysis shown in (A).-Intensity of fluorescence was determined using Image J program. Error bars represent SD (n = 3). Asterisks show statistical significance or p < 0.0001 in a t-test. (C) Fluorescence micrographs of tumor sections 20 min after injection with IF7-A488 (a) or RQ7-A488 (b), coinjected with Texas-red conjugated tomato lectin. Shown are IF7-A488 or RQ7-A488 (left), Texas-red tomato lectin (middle), and the merge with DAPI nuclear staining (right). (Scale bar,50 μm).