Fig. 3.

Changes in mucosal cytokine production and the integrity of intestinal barrier function in low-PC mice. (A and B) Secretion of IL-6, KC, and macrophage inflammatory protein 2 by the mucosa of the colon (A) and TEER measured from the colon (B) of low-PC and WT mice on days 0, 3, and 7 of DSS administration. (C and D) Immunofluorescent staining and bar graph showing the expression of JAM-A, CL-3, CL-10, and ZO-1 tight junction proteins (green) in the colon of low-PC and WT mice before DSS administration (day 0); nuclei were stained with DAPI (blue). (Scale bar: 30 μm.) Values represent mean ± SEM; n = 13 for WT mice and n = 20 for low-PC mice. These data are representative of three independent experiments. *P < 0.05; **P < 0.01.