Fig. 2.

BjPCR1 antisense lines exhibited reduced translocation of Ca²⁺ to the shoot and reduced radial translocation of Ca²⁺ to the inner part of the root. Ca (A) and Zn (B) concentrations in the shoots and roots of 4-wk-old WT and anti-BjPCR1 B. juncea plants. DW, dry weight. (C) Shoot-to-root ratios of Ca and Zn concentrations as shown in A and B. (D) Autoradiography of 3-wk-old B. juncea plants incubated in hydroponic medium supplemented with 1.5 mM CaCl₂ containing 0.4 MBq of ⁴⁵CaCl₂ supplied through the root for 15 h. (E) Counts of ⁴⁵Ca normalized by the volume of cell sap extracted from the shoot of plants treated with ⁴⁵CaCl₂ as in D. All data represent average ± SE (n = 5, N = 2). (F) Distribution of free Ca²⁺ in the roots of the WT and anti-BjPCR1 lines visualized using fluo-3 fluorescence. Roots of 5-d-old B. juncea plants were stained with fluo-3 for 4 h, washed with PBS solution, and observed by confocal microscopy. (Scale bar = 500 μm.)