Formation of the inflammasome in the granulation tissue and cardiomyocytes. Immunofluorescence shows overlap of cardiac actin (green) and the different components of the inflammasome [ASC (red, A–D), cryopyrin (red, E–H), and caspase-1 (red, I–L)] in the granulation tissue and border zone, 3 and 7 d after AMI (C, G, and K and D, H, and L, respectively), with minimal staining and overlap in the sham-operated mouse or the remote zone (A, E, and I and B, F, and J, respectively). Counterstaining with DAPI (blue). Original magnification 40×. (Scale bar, 20 μm.)