Fig. 3.

FIT2 exhibits lipid binding specificity. (A) Competition binding assay. FIT2-His₆-StrepII was incubated with [9,10-³H(N)]-triolein in the absence or presence of the indicated excess unlabeled lipid. Data are represented as mean ± SD. These results are representative of five experiments. (NC, no competitor; TAG, triolein; DAG, 1-palmitoyl-2-oleoyl-sn-glycerol; CO, cholesteryl oleate). (B) Direct binding of FIT2-His₆-StrepII to [1,2,3-³H]-dioleoyl-rac-glycerol ([³H]-DAG) and competition by unlabeled 1-palmitoyl-2-oleoyl-sn-glycerol. Data are represented as mean ± SD. This experiment was replicated twice with similar results.