Fig. 6.

Impaired H3 removal upon transcriptional activation. (A) ChIP analysis of Yta7-TAP across the inducible FMP27 allele in inducing and non-inducing conditions. The two conditions represent YPRaffinose (2%, non-inducing) and the same cultures 1 h after addition of galactose. Values were obtained with qPCR and represent the IP signal over the IP signal for the negative internal control PRP8. (B) H3 (Left) and Rpb3-HA (Right) ChIP performed in parallel on the same sonicated chromatin samples. Represented values are IP/IN, adjusted to a WT level set equal to 1 for each primer set. Raw IP/IN values for Rpb3-HA ChIP in both WT and the yta7mutant were relatively uniform (±15%) across the locus. Distances given are with respect to the start of the ORF.