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Quantification of protein immobilization. (A) TEM images showing the association of 1.4-nm AuNPs labels with the SPBCL-defined large AuNPs. The number of the AuNP labels and, in turn the number of the immobilized streptavidin molecules is dependent on the size of the SPBCL-defined AuNPs. (B) Histogram of the number of the associated small AuNP labels as a function of the size of the AuNPs patterned by SPBCL.
