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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Oct 25;44(1):158–169. doi: 10.1016/j.biocel.2011.10.011

Figure 1. ET-1 induces FAK activation in normal lung fibroblasts.

Figure 1

(A) Growth-arrested normal human lung fibroblasts (IMR-90) were treated with ET-1 at doses ranging from 1 pM (0.001 nM) to 1 μM (103 nM). At 30 minutes, cell lysates were assessed for FAK activation by Western blotting for phosphorylation at tyrosine-397 (Y397 p-FAK). The blot was stripped and probed for total FAK. (B) Densitometric analysis of (A) normalized to untreated controls. * p < 0.01 compared to untreated controls; n = 3. (C) IMR-90 fibroblasts were treated with ET-1 (1.0 nM) for the time periods indicated and assessed for FAK activation by Western blotting for Y397 p-FAK. The blots were stripped and probed for total FAK. (D) Densitometric analysis of (C) normalized to untreated controls. * p < 0.01 compared to untreated controls; n = 3.