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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Oct 25;44(1):158–169. doi: 10.1016/j.biocel.2011.10.011

Figure 5. FRNK overexpression attenduates ET-1 mediated protection from apoptosis.

Figure 5

IMR-90 fibroblasts were infected with adenoviral vectors to overexpress either green fluorescent protein (GFP) or FAK-related non-kinase (FRNK) with a multiplicity of infection (MOI) of 40. (A) Infected cells were treated with/without ET-1 (1.0 nM) for 1 hour, and whole cell lysates were assessed for FAK phosphorylation (upper blot). The blot was stripped and probed for Total FAK (lower blot). In the lower blot, the truncated protein (FRNK) can be appreciated as the low molecular weight band. (B) Infected cells were treated for 16 hours with/without ET-1 (1.0 nM) and/or Fas-activating ligand (CH11; 250 ng/ml) in the presence/absence of CHX (500 ng/ml). Apoptosis was assessed by Western blotting to detect cleaved PARP. (C) GFP-infected cells (white bars) and FRNK-infected cells (black bars) were treated with/without the combination of Fas/CHX for 16 hours in the presence/absence of ET-1. Apoptosis was assessed by ELISA detection of histone-associated DNA fragments, and the data were normalized to the GFP-infected cells treated with Fas/CHX. * p < 0.01 vs. Fas/CHX-treated GFP-infected cells. # = p < 0.01 vs. GFP infected cells treated with Fas/CHX with ET-1. NS = non-significant differences between the indicated groups; n = 3.