Skip to main content
. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Oct 25;44(1):158–169. doi: 10.1016/j.biocel.2011.10.011

Figure 7. Survivin upregulation by ET-1 is mediated by ROCK, FAK and PI3K/AKT.

Figure 7

(A) IMR-90 fibroblasts were treated with ET-1 (1.0 nM) for the indicated time points and Survivin expression was measured by Western blotting. Blots were stripped and probed for GAPDH. (B) Densitometric analysis of Survivin expression.* p < 0.01 vs. untreated controls; n = 4. (C) Fibroblasts were treated with/without ET-1 (1.0 nM) for 6 hours in the presence/absence of inhibitors of ROCK (Y27632, 15 nM) or FAK (PF573228, 10 μM). Survivin expression was assessed by Western blotting. Blots were stripped and probed for GAPDH. (D) Fibroblasts were treated with ET-1 (1.0 nM) for 6 hours in the presence/absence of two different PI3K inhibitors (LY294002; 10 μM or Wortmannin 50 nM). Survivin expression was assessed by Western blotting and blots were stripped and probed for GAPDH. (E) Densitometric analysis of Survivin expression in the presence/absence of the inhibitors used in (C and D), normalized to untreated controls. * p < 0.001 vs. untreated controls; # p < 0.01 vs. ET-1 treated cells. n = 3 per condition.