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. Author manuscript; available in PMC: 2012 Dec 13.
Published in final edited form as: Dev Cell. 2011 Nov 23;21(6):1062–1076. doi: 10.1016/j.devcel.2011.09.014

Figure 1. Sag inactivation disrupts vascular development in yolk-sac and embryo brains.

Figure 1

(A) Appearance of mouse embryos at E10.5 with (left) and without (right) yolk-sacs. Arrow points to pericardial edema. Bar size=0.75 mm. (B) Appearance of mouse yolk sac at E10.5 after whole-mount staining with CD31. Bar size=1 mm. (C&D) Appearance of mouse embryo brain regions at E10.5 after whole-mount CD31 staining of embryos. Bar size = 3 mm. (E) Reduced number of blood vessles in Sag−/− embryos: Haematoxylin and eosin stained embryo sections of Sag+/+ (a, c) and Sag−/− (b,d) at E10.5. * indicates network of small blood vessels with cellular contents. Arrow heads indicate blood vessels (c, d) around brain region. Inserts indicate number and size of blood vessels (*) within neuroepithelium. Number of blood vessels around the entire brain region was counted (bars, s.e.m., n=5 counted areas). (F) Data was plotted. **, p=0.00269. See also Figure S1.