Figure 4. Gene delivery of IL-10 enhances neurogenesis in SGZ of APP+PS1 mice.
(a–c) Representative images of Dcx staining in the dentate gyrus of non-Tg (a) or APP+PS1 mice injected with AAV-GFP (b) or AAV-IL-10 (c) into the hippocampus at 3 months of age and i.p. injected with BrdU 3 weeks prior to the euthanasia at 8 months of age. Scale bar, 200µm. (d–f) Immunofluorescence of BrdU (green) and NeuN (red) in the dentate gyrus of non-Tg (d) or APP+PS1 mice injected with AAV-GFP (e) or AAV-IL-10 (f). Scale bar, 100µm. (g, h, i) Quantification of Dcx+ (g), BrdU+ cells (h), or BrdU+NeuN+ cells (i) in SGZ. Bars represent mean ± SEM (n = 5 per group, 10 sections per brain). ** or *** denotes P <0.01 or 0.001 versus non-Tg, and # or ## denotes P < 0.05 or 0.01 versus AAV-GFP group, as determined by one-way ANOVA and Newman–Keuls post-test.