Figure 4.
P. vulgaris (green bean) maceration by P. syringae. Green beans were inoculated with 10 μl of an overnight culture (~1×105 CFU/ml) of wild-type P. syringae or P. syringae (ΔahlI) and the indicated concentration of AHL, and incubated for 72 h (see Methods). (A) Image of green beans inoculated with (left to right): (1) wild-type P. syringae, (2) P. syringae (ΔahlI), (3) buffer alone. (B) Maceration of green bean with wild-type P. syringae (grey bar) or with P. syringae (ΔahlI) and varying concentrations of OHHL (5) (white bars). Results are expressed as the average macerated mass +/− the standard error of the mean (n=9).
